abstract：:The induction of paraffin oxidation in intact cells of Pseudomonas aeruginosa was investigated. Oxidation of (14)C-heptane by cell-free extracts of adapted cells showed that the activity of whole cells is a reliable reflection of the synthesis of the first enzyme in the degradation of n-alkanes. Induction was signific...

journal_title：Journal of bacteriology

authors： van Eyk J,Bartels TJ

更新日期：1968-09-01 00:00:00

abstract：:Cells of Bacillus thuringiensis containing refractile spores autolyzed readily when suspended in buffer. The autolysate contained enzymes which lysed vegetative cell walls of the organism. Three enzymes were isolated from the autolysate, and each was purified approximately 30-fold. One enzyme, most active near pH 4.0,...

journal_title：Journal of bacteriology

authors： Kingan SL,Ensign JC

更新日期：1968-09-01 00:00:00

abstract：:Approximately 2 x 10(11) viable Pasteurella tularensis cells per ml, contained in suspensions, were killed by exposure to 10(6) r of gamma-radiation. When injected intraperitoneally into mice, the irradiated suspensions initially contained about 10 ld(50) per ml, and immunized mice against challenge with fully virulen...

journal_title：Journal of bacteriology

authors： Landay ME,Wright GG,Pulliam JD,Finegold MJ

更新日期：1968-09-01 00:00:00

abstract：:Experiments were performed to characterize the pyrogenic principle of ribonucleic acid (RNA) from the yeast Candida utilis. It was shown that ribonuclease hydrolysis of the RNA does not lead to inactivation of the pyrogenicity. Pyrogenicity was, however, destroyed by treatment with sodium deoxycholate. On column chrom...

journal_title：Journal of bacteriology

authors： Kanoh S,Kohlhage H,Siegert R

更新日期：1968-09-01 00:00:00

abstract：:Several preparations of staphylococcal alpha-toxin and delta-lysin were studied in order to compare hemolytic activity with capacity to lyse bacterial protoplasts. delta-Lysin in relatively low concentration lysed protoplasts of Sarcina lutea, protoplasts of Streptococcus faecalis, and spheroplasts of Escherichia coli...

journal_title：Journal of bacteriology

authors： Bernheimer AW,Avigad LS,Grushoff P

更新日期：1968-08-01 00:00:00

abstract：:The enzyme pattern of Saccharomyces cerevisiae was followed during batch growth and in continuous culture in a synthetic medium limited for glucose under aerobic conditions. Seven enzymes were measured: succinate-cytochrome c oxidoreductase, malate dehydrogenase, nicotinamide adenine dinucleotide-linked glutamate dehy...

journal_title：Journal of bacteriology

authors： Beck C,von Meyenburg HK

更新日期：1968-08-01 00:00:00

abstract：:The growth constant and Y (sucrose) (grams of cells per mole of sucrose) for NH(3)-grown cultures of Clostridium pasteurianum were 1.7 times those of N(2)-grown cultures, whereas the rate of sucrose utilized per gram of cells per hour was similar for both conditions. The Y (sucrose) of chemostat cultures grown on limi...

journal_title：Journal of bacteriology

authors： Daesch G,Mortenson LE

更新日期：1968-08-01 00:00:00

abstract：:Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%...

journal_title：Journal of bacteriology

authors： Friedberg I,Avigad G

更新日期：1968-08-01 00:00:00

abstract：:Ultraviolet (UV) microscopy was used to study the capacity of yeast (ATCC 10231 and 10261) and filamentous (ATCC 10259) strains of Candida albicans to accumulate UV-absorbing materials from a medium supplemented with purines, pyrimidines, amino acids, or related compounds as the main nitrogen source. All strains accum...

journal_title：Journal of bacteriology

authors： Balish E,Svihla G

更新日期：1968-07-01 00:00:00

abstract：:The leucine auxotroph Escherichia coli 2961 exhibited stringent control of net ribonucleic acid (RNA) synthesis during amino acid starvation. After leucine was exhausted from the medium, the rate of uracil incorporation into RNA rapidly decreased to 2 to 4% of the prestarvation value. Infection of the starved cells wi...

journal_title：Journal of bacteriology

authors： Sharp K,Green MH

更新日期：1968-07-01 00:00:00

abstract：:The model of pneumonia produced by intranasal inoculation of Mycoplasma pulmonis in gnotobiotic mice provided the opportunity to study the localization and identification of the infecting organisms in the tissues by immunofluorescence techniques. Frozen sections of pneumonic mouse lung were fixed in acetone, layered w...

journal_title：Journal of bacteriology

authors： Organick AB,Lutsky II

更新日期：1968-07-01 00:00:00

abstract：:Circulating endotoxin was specifically precipitated from plasma samples withdrawn from three different animal species subsequent to parenteral injection of the toxin. Lipoprotein-positive staining and esterase activity were demonstrated on the precipitation lines formed in immunodiffusion, thus establishing the in viv...

journal_title：Journal of bacteriology

authors： Skarnes RC

更新日期：1968-06-01 00:00:00

abstract：:Mutants of Agrobacterium tumefaciens requiring methionine for growth on a solid basal medium were induced by the use of N-methyl-N'-nitro-N-nitrosoguanidine. In addition to the difference of mutant strains, the extent of methionine dependency differed in a liquid basal medium and in the presence of aspartate or fumara...

journal_title：Journal of bacteriology

authors： Kaneshiro T

更新日期：1968-06-01 00:00:00

abstract：:Pulse labeling of deoxyribonucleic acid (DNA) and radioautography have been used to study the effect of growth rate on nuclear replication in Aspergillus nidulans. When conidia were germinated in media supporting a fast growth rate, the radioactive pulse labeled either all of the nuclei in a cell or none of them. At s...

journal_title：Journal of bacteriology

authors： Kessel M,Rosenberger RF

更新日期：1968-06-01 00:00:00

abstract：:Median longitudinal sections of germ tube apices of Mucor rouxii revealed the presence of a single, roughly hemispherical, electron-dense organelle, in intimate contact with the apical cell wall. Conceivably, this "apical corpuscle" may be responsible for the emission of the germ tube and its continued apical growth. ...

journal_title：Journal of bacteriology

authors： Bartnicki-Garcia S,Nelson N,Cota-Robles E

更新日期：1968-06-01 00:00:00

abstract：:The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-...

journal_title：Journal of bacteriology

authors： Previte JJ

更新日期：1968-06-01 00:00:00

abstract：:Passive cutaneous anaphylaxis (PCA) was produced in guinea pigs sensitized with guinea pig Coxiella burneti phase I-II antiserum and challenged with dimethylsulfoxide- or trichloroacetic acid-soluble extracts from phase I cells. The PCA reaction could not be induced by whole or mechanically disrupted phase I or phase ...

journal_title：Journal of bacteriology

authors： Peacock M,Munoz J,Tallent GL,Ormsbee RA

更新日期：1968-05-01 00:00:00

abstract：:The accumulation of imidazoleglycerol phosphate during growth of Neurospora crassa in the presence of 3-amino-1,2,4-triazole was found to cause derepression of tryptophan synthetase and to inhibit the induction of kynureninase. Accumulation of indoleglycerol phosphate in response to growth in the presence of indole ac...

journal_title：Journal of bacteriology

authors： Turner JR,Matchett WH

更新日期：1968-05-01 00:00:00

abstract：:The inducible galactose transport system in bakers' yeast carries out the facilitated diffusion of the nonmetabolized galactose analogues d-fucose and l-arabinose. This capacity depends on the activity of the Ga 2 gene. In some strains, d-fucose and l-arabinose are also gratuitous inducers. Mutants in which the induci...

journal_title：Journal of bacteriology

authors： Cirillo VP

更新日期：1968-05-01 00:00:00

abstract：:Three floc-forming, gram-negative, polarly flagellated rods were isolated and characterized. Our isolates were compared to four similar floc-forming organisms previously isolated in another laboratory and classified as two species of Zoogloea, one of Pseudomonas, and as one unidentified gram-negative rod. Possession o...

journal_title：Journal of bacteriology

authors： Friedman BA,Dugan PR

更新日期：1968-05-01 00:00:00

abstract：:The methylenetetrahydrofolate dehydrogenase of the amethopterin-resistant strain Streptococcus faecium var. durans A(k) was purified 100-fold. Because it is extremely labile, this enzyme required protection by 1 mm nicotinamide adenine dinucleotide phosphate (NADP(+)) during purification; 0.01 mm EADP(+) with 0.1% bov...

journal_title：Journal of bacteriology

authors： Albrecht AM,Pearce FK,Hutchison DJ

更新日期：1968-05-01 00:00:00

abstract：:Unsaturated fatty acids having structural features which are different from those of the monoenoic acids normally synthesized by Escherichia coli can serve as growth factors for an auxotroph requiring unsaturated fatty acids. These analogues were incorporated into the phospholipids, as shown by gas-liquid and thin-lay...

journal_title：Journal of bacteriology

authors： Silbert DF,Ruch F,Vagelos PR

更新日期：1968-05-01 00:00:00

abstract：:The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C...

journal_title：Journal of bacteriology

authors： Duncan CL,Sugiyama H,Strong DH

更新日期：1968-05-01 00:00:00

abstract：:We investigated the effect of time, temperature, and the presence of sodium chloride, nitrates, and nitrites in the medium on the growth and production of enterotoxin B by Staphylococcus aureus. Assays by the double gel-diffusion method showed that maximal enterotoxin B production occurs at the beginning of the statio...

journal_title：Journal of bacteriology

authors： McLean RA,Lilly HD,Alford JA

更新日期：1968-04-01 00:00:00

abstract：:Auxotrophic mutants of Hydrogenomonas eutropha and H. facilis requiring utilizable amino acids were employed to demonstrate the simultaneous utilization of H(2) and an organic substrate for growth. The ratio of the cell yields under dual substrate conditions compared to heterotrophic conditions indicated the relative ...

journal_title：Journal of bacteriology

authors： DeCicco BT,Stukus PE

更新日期：1968-04-01 00:00:00

abstract：:Since relatively few quantitative immunological studies had been conducted with invertebrates, this study was designed to learn more about the immune potential of the oyster, a molluscan representative. Experiments measured the primary and secondary clearance rates of T2 coliphage in the oyster. The fate of phage inje...

journal_title：Journal of bacteriology

authors： Acton RT,Evans EE

更新日期：1968-04-01 00:00:00

abstract：:No catalase activity was detected in four strains of glucose-grown Mycoplasma pneumoniae at any time during the replication of the organism. Exogenous catalase dramatically increased the O(2) uptake with glycerol, presumably by releasing inhibition caused by hydrogen peroxide. The effect of added catalase on the O(2) ...

journal_title：Journal of bacteriology

authors： Low IE,Eaton MD,Proctor P

更新日期：1968-04-01 00:00:00

abstract：:Previous investigations have demonstrated the existence of five Histoplasma capsulatum serotypes. Available specific fluorescent-antibody reagents stain only four of the five serotypes. Antibodies produced against the most complete H. capsulatum serotype were labeled with fluorescein isothiocyanate to develop a reagen...

journal_title：Journal of bacteriology

authors： Kaufman L,Blumer S

更新日期：1968-04-01 00:00:00

abstract：:A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These "lighter" bac...

journal_title：Journal of bacteriology

authors： Cahn FH,Fox MS

更新日期：1968-03-01 00:00:00

abstract：:We investigated an osmotic remedial mutant of Saccharomyces which was deficient in galactose-1-phosphate uridyl transferase. Both the rate of growth and the transferase activity of the mutant culture were dependent on the osmotic activity of the growth medium. Organic and ionic solutes proved to be equally effective i...

journal_title：Journal of bacteriology

authors： Bassel J,Douglas HC

更新日期：1968-03-01 00:00:00

abstract：:The mutant am-14 produces no active nicotinamide adenine dinucleotide phosphate-linked glutamate dehydrogenase (GDH) and no protein showing immunological cross-reaction with the enzyme. Nevertheless, it shows complementation with several other am mutants in heterokaryons. Active GDH can be extracted from heterokaryons...

journal_title：Journal of bacteriology

authors： Sundaram TK,Fincham JR

更新日期：1968-03-01 00:00:00

abstract：:The cell wall lipopolysaccharides from 17 species belonging to the genus Xanthomonas were extracted from the cells with hot 45% phenol. After purification, the components of the polysaccharide were obtained by acid hydrolysis of the lipopolysaccharide and were quantitatively assayed. Data obtained show that all prepar...

journal_title：Journal of bacteriology

authors： Volk WA

更新日期：1968-03-01 00:00:00

abstract：:The effects of starving Escherichia coli for glucose and required amino acids were determined. The disappearance of the majority of the ribosomal population, particularly of the ribonucleic acid (RNA) moiety, was noted. The events during recovery are detailed, with emphasis placed on the timing, requirements, and cont...

journal_title：Journal of bacteriology

authors： Jacobson A,Gillespie D

更新日期：1968-03-01 00:00:00

abstract：:Seven mutants of Haemophilus influenzae were isolated by the criterion of sensitivity to ultraviolet (UV) inactivation of colony formation. These mutants and the wild type were characterized with regard to X-ray inactivation of colony formation, UV induction of division inhibition, the ability of the eight strains to ...

journal_title：Journal of bacteriology

authors： Setlow JK,Brown DC,Boling ME,Mattingly A,Gordon MP

更新日期：1968-02-01 00:00:00

abstract：:A Boivin preparation of Brucella abortus, unlike common enterobacterial endotoxins, failed to depress water intake or increase numbers of hemolysin-producing spleen cells in mice, or to cause delayed inflammatory reactions in rabbit skin. Reactivity to the B. abortus endotoxin was found only in animals which were prev...

journal_title：Journal of bacteriology

authors： Freedman HH,Fox AE,Willis RS,Schwartz BS

更新日期：1968-02-01 00:00:00

abstract：:An attempt was made to explain the puzzling observation that in bacteria 2,6-diaminopurine can replace guanine for guanineless mutants and for xanthineless mutants (both of which can make adenosine monophosphate de novo) but not for nonexacting purine auxotrophs (which cannot make adenosine monophosphate de novo). The...

journal_title：Journal of bacteriology

authors： Demain AL,Shigeura HT

更新日期：1968-02-01 00:00:00

abstract：:Mated cultures of Blakeslea trispora, grown in a potato extract-glucose-thiamine medium, produced 10 to 15 times more beta-carotene than either unmated culture. Mated, but not unmated, cultures produced a family of compounds (beta factor) which stimulated carotenogenesis in unmated cultures. In fact, carotenogenesis w...

journal_title：Journal of bacteriology

authors： Sutter RP,Rafelson ME Jr

更新日期：1968-02-01 00:00:00

abstract：:Salmonella typhimurium was found to utilize 2-deoxyribose as a sole carbon and energy source. Cells grown in the presence of deoxyribose contained increased levels of deoxyribose kinase, thymidine phosphorylase, and two forms of deoxyribose-5-phosphate aldolase (DR5P aldolase). One form of DR5P aldolase was induced by...

journal_title：Journal of bacteriology

authors： Hoffee PA

更新日期：1968-02-01 00:00:00

abstract：:Mycelial mats of Ascodesmis sphaerospora were fixed and embedded for electron microscopy, and thin sections of 1-mm blocks, taken from the 1st to the 7th mm behind the hyphal tips, were cut parallel to the long axis of the hyphae. The hyphal tip region is characterized by an outer zone of electron-transparent vesicles...

journal_title：Journal of bacteriology

authors： Brenner DM,Carroll GC

更新日期：1968-02-01 00:00:00

abstract：:Several resistant mutants of a transformable group H streptococcus, strain Challis, were isolated from media containing high concentrations of streptomycin. Mutants SR5a and SR5 exhibited high and low transformability, respectively, when exposed to deoxyribonucleic acid (DNA) from a novobiocin-resistant Challis strain...

journal_title：Journal of bacteriology

authors： Perry D

更新日期：1968-01-01 00:00:00